Histone H3 Mouse mAb-科鹿（武漢）生物科技有限責任公司

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Histone H3 Mouse mAb

EM1037

規格：	價格：
50 μl	￥1280.00
100 μl	￥1980.00

下載說明 ①

Overview

Product name:	Histone H3 Mouse mAb
Human Swiss-Prot No.:	P68431
Reactivity:	Human, Mouse, Rat, Yeast
Alternative Names:	H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Source:	Mouse
Applications :	WB, IHC, IF, IP
Species reactivity:	Human, Mouse, Rat, Yeast
Species:	Human
Recommended dilutions:	WB 1:2,000-5,000 IP 1:200 IF 1:200
Immunogen:	Recombinant Protein
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid?repeated?freeze-thaw?cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/ml
Observed band:	15kDa
GeneID（Human）:	8290
Cellular localization:	Nucleus,Chromosome
Background:	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with EM1037 diluted at 1:5,000.
IF analysis of Hela with DAPI (Left) and EM1037(Right) diluted at 1:200.
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1037 1:2,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000
Western blot analysis of?extracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Brain (Lane 4), using Histone H3 diluted?at?1:5,000.

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